Other

Part:BBa_K3228101

Designed by: Marian Krämer   Group: iGEM19_Marburg   (2019-10-18)


pMC_0_3-5_1_crRNA-GFP_dropout

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1186
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1186
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1186
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 242

Description

This part has a GFP spaceholder for the gRNA of the crRNA of the CRISPR/cpf1 system. It is used to insert a gRNA for targeted knockouts in the CRISPR/cpf1 system of iGEM Marburg 2019.

Compability

These parts are compatible with the RCF [1000] standard. These parts are compatible with any part collection that uses the PhytoBrick standard of overhangs. For more information we recommend to head over to Design of the Marburg Collection iGEM Marburg 2018.

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